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How to give a shot of testosterone: 14 steps (with pictures), How to give a shot of testosterone two parts: deciding whether testosterone therapy is appropriate performing a testosterone injection community q&a testosterone is a hormone produced in the testicles in men and in the ovaries in women.. Male hormone restoration - testosterone, estrogen, The patterns and trends over time of multiple hormone levels, (for instance free testosterone, total testosterone, and estrogen), determine the specific hormone replacements required. it may not be safe to use large amounts of testosterone in any form without also using aromatase-inhibiting supplements or medications..